Detection of IgG rheumatoid factor by concanavalin A treatment and complement fixation with IgG rheumatoid factor.
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چکیده
منابع مشابه
Complement fixation by rheumatoid factor.
The capacity for fixation and activation of hemolytic complement by polyclonal IgM rheumatoid factors (RF) isolated from sera of patients with rheumatoid arthritis and monoclonal IgM-RF isolated from the cryoprecipitates of patients with IgM-IgG mixed cryoglobulinemia was examined. RF mixed with aggregated, reduced, and alkylated human IgG (Agg-R/A-IgG) in the fluid phase failed to significantl...
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Sir: Winchester et al two decades ago proposed that IgG rheumatoid factor (RF) could be locally produced in plasma cells that line the synovium.' We conducted a short study looking at paired samples of synovial fluid (from the knee joint) and serum from patients with definite rheumatoid arthritis to determine if the production of IgG RF is localised in the synovial fluid. Thirty six serum sampl...
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Serum samples from patients with seropositive rheumatoid arthritis contain an inhibitor of complement mediated inhibition of immune precipitation (CMIP). This inhibitory effect can be produced by the addition of either purified monoclonal or polyclonal IgM rheumatoid factor (RF) to human serum. The specificity of the rheumatoid factor influences the degree of inhibition, and when precipitation ...
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A sensitive radioimmunoassay has been applied to the study of rheumatoid factor activity of IgM and IgG subclasses in serum and synovial fluid of patients with rheumatoid arthritis. Although rheumatoid factor activity was found in all four subclasses, its distribution was not the same as that of the total subclasses in normal sera. Results are discussed in relation to the possible clinical valu...
متن کاملNegative complement-fixation tests with rheumatoid factor positive sera. A simple method for selective removal of rheumatoid factor.
Sera from patients with rheumatoid arthritis regularly contain inhibitors of complement fixation (CF) to antigen-antibody complexes (Heimer and Levin, 1965). Removal of rheumatoid factor (RF) by absorption of sera with heat-aggregated human y-globulin resulted in a higher number of positives and a rise in CF antibody titre against antigens from herpes simplex, mumps, and morbilli viruses (Stanf...
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 1976
ISSN: 0003-4967
DOI: 10.1136/ard.35.3.240